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naïve cd4 t cell isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec naïve cd4 t cell isolation kit
A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from <t>CD4-Cre</t> Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify <t>CD4</t> <t>T</t> cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.
Naïve Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/naïve cd4 t cell isolation kit/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
naïve cd4 t cell isolation kit - by Bioz Stars, 2026-03
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naïve cd4 t cells isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec naïve cd4 t cells isolation kit
A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from <t>CD4-Cre</t> Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify <t>CD4</t> <t>T</t> cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.
Naïve Cd4 T Cells Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/naïve cd4 t cells isolation kit/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
naïve cd4 t cells isolation kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
mouse naïve cd4 t cell isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec mouse naïve cd4 t cell isolation kit
A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from <t>CD4-Cre</t> Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify <t>CD4</t> <t>T</t> cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.
Mouse Naïve Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse naïve cd4 t cell isolation kit/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
mouse naïve cd4 t cell isolation kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
naïve cd4 t cells  (Miltenyi Biotec)
99
Miltenyi Biotec naïve cd4 t cells
A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from <t>CD4-Cre</t> Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify <t>CD4</t> <t>T</t> cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.
Naïve Cd4 T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/naïve cd4 t cells/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
naïve cd4 t cells - by Bioz Stars, 2026-03
99/100 stars
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A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from CD4-Cre Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify CD4 T cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.

Journal: bioRxiv

Article Title: Tissues Guide Dependence of Treg on the Transferrin Receptor

doi: 10.64898/2026.02.01.703140

Figure Lengend Snippet: A) To test Treg suppressive function in vivo , WT and KO Tregs isolated from CD4-Cre Tfrc floxed mice were directly compared in a colitis model. B) Body weight of recipient mice was monitored during disease development until the humane end point when the first mouse had lost more than 20% of its original body weight. Average weight loss of all mice in each group is shown. C) Colons were processed for H&E staining and pathology scores were determined by a blinded veterinary pathologist for the proximal (D), middle (E), and distal (F) regions of the colon. Data are from 2 independent experiments. One-way ANOVA with Sidak’s multiple comparisons test. G) Mesenteric lymph nodes (mLN) were processed for flow cytometry to analyze the frequencies of transferred Tregs, their Foxp3 expression (H), and Helios expression (I). J) One representative tail from both recipient mice of the WT and KO Tregs. K) Ears of recipient mice were processed for flow cytometry to count Tregs (K), Foxp3 expression (L), and quantify CD4 T cells in the skin (M). (K-M) Unpaired two-tailed Student’s t-test.

Article Snippet: WT and KO Tregs were isolated from CD4-cre + and – spleens according to the (Stem Cell Technologies) and mixed in a 1 to 3 ratio with naïve CD4 T cells isolated from WT CD45.1 mice according to the Naïve CD4 T Cell Isolation Kit (Miltenyi Biotec).

Techniques: In Vivo, Isolation, Staining, Flow Cytometry, Expressing, Two Tailed Test

A) Representative images of Foxp3 YFP -Cre, Tfrc floxed mice with scaly skin on the tail and paws of KO mice. B) Representative images of H&E-stained ears from littermates. Scale bar= 0.10 mm. C) Ears were processed into single cell suspensions and CD4+ and cytotoxic CD8+ T cells quantified by flow cytometry. D) IFNy+ CD4 T cells quantified by flow cytometry in the skin samples. E-G) Increased infiltration of macrophages (CD11b+F4/80+), IL-17a⁺ CD4 T cells, and neutrophils (CD11b+Ly6G+) in KO ears. H) H&E-stained lung sections. Scale bar = 0.5 mm, inset scale bar= 0.1 mm. (I) Lungs were processed into single cell suspension to quantify CD4 and CD8 T cells by flow cytometry. J) Frequency of T-bet+ Th1 cells in lungs, IFN-y producing T cells and TNF-a+ T cells. K) Th2-associated populations, Gata-3+ Th2 cells, IL-13 and IL-5 cells. Frequency of Th17 cells (L) and Foxp3+ Tregs (M) in the lungs. All statistical tests are unpaired two-tailed Student’s t-test.

Journal: bioRxiv

Article Title: Tissues Guide Dependence of Treg on the Transferrin Receptor

doi: 10.64898/2026.02.01.703140

Figure Lengend Snippet: A) Representative images of Foxp3 YFP -Cre, Tfrc floxed mice with scaly skin on the tail and paws of KO mice. B) Representative images of H&E-stained ears from littermates. Scale bar= 0.10 mm. C) Ears were processed into single cell suspensions and CD4+ and cytotoxic CD8+ T cells quantified by flow cytometry. D) IFNy+ CD4 T cells quantified by flow cytometry in the skin samples. E-G) Increased infiltration of macrophages (CD11b+F4/80+), IL-17a⁺ CD4 T cells, and neutrophils (CD11b+Ly6G+) in KO ears. H) H&E-stained lung sections. Scale bar = 0.5 mm, inset scale bar= 0.1 mm. (I) Lungs were processed into single cell suspension to quantify CD4 and CD8 T cells by flow cytometry. J) Frequency of T-bet+ Th1 cells in lungs, IFN-y producing T cells and TNF-a+ T cells. K) Th2-associated populations, Gata-3+ Th2 cells, IL-13 and IL-5 cells. Frequency of Th17 cells (L) and Foxp3+ Tregs (M) in the lungs. All statistical tests are unpaired two-tailed Student’s t-test.

Article Snippet: WT and KO Tregs were isolated from CD4-cre + and – spleens according to the (Stem Cell Technologies) and mixed in a 1 to 3 ratio with naïve CD4 T cells isolated from WT CD45.1 mice according to the Naïve CD4 T Cell Isolation Kit (Miltenyi Biotec).

Techniques: Staining, Single Cell, Flow Cytometry, Suspension, Two Tailed Test

WT mice were treated with ethanol (EtOH) or MC903 for 10 days to induce atopic dermatitis (AD) on the ears. A) Frequency of Foxp3+ cells of CD45+CD4+ cells in the ears of WT mice. B) Flow cytometric quantification of geometric MFI (gMFI) of CD71 expression on Tregs from the ears or pLN of WT mice. C) BioTracker Labile Iron dye quantification of free iron in live GFP+ Tregs from the ears of WT mice. D) Foxp3-EGFP-ER T2 -Cre mice were treated with tamoxifen or corn oil injections to induce deletion of Tfrc and subsequently treated with ethanol (EtOH) or MC903 for 10 days. E) On day 11, MC903 treated mice were blindly scored for scratching behavior for 10 minutes each. F) Representative images of ears from each treatment group (left). Disease severity scores were assessed by a blinded dermatologist based on Physician global assessment (PGA) score. G) Representative HE images of ears. Scale bar = 500 μm. H) Total CD45+ cells in the ears were counted by flow cytometry. I) Eosinophil counts defined as total live single cell CD45+ CD11b+ Ly6G- Siglec F+ cells. J) Monocyte counts defined as total live single cell CD45+ CD11b+ Ly6G- Siglec F- F480- CD11c-. K) CD4 T cell frequencies in MC903 treated mouse ears. L) Treg frequencies in the ears and (M) pLN. N) Free iron was quantified by flow cytometry in live GFP+ Tregs from the pLN. O) Frataxin expression of Foxp3+ Tregs was determined by flow cytometry in the pLN. A, C, E, G, H, M= Welch’s t test. B, H, I, J, L, M N, O= 2 way ANOVA.

Journal: bioRxiv

Article Title: Tissues Guide Dependence of Treg on the Transferrin Receptor

doi: 10.64898/2026.02.01.703140

Figure Lengend Snippet: WT mice were treated with ethanol (EtOH) or MC903 for 10 days to induce atopic dermatitis (AD) on the ears. A) Frequency of Foxp3+ cells of CD45+CD4+ cells in the ears of WT mice. B) Flow cytometric quantification of geometric MFI (gMFI) of CD71 expression on Tregs from the ears or pLN of WT mice. C) BioTracker Labile Iron dye quantification of free iron in live GFP+ Tregs from the ears of WT mice. D) Foxp3-EGFP-ER T2 -Cre mice were treated with tamoxifen or corn oil injections to induce deletion of Tfrc and subsequently treated with ethanol (EtOH) or MC903 for 10 days. E) On day 11, MC903 treated mice were blindly scored for scratching behavior for 10 minutes each. F) Representative images of ears from each treatment group (left). Disease severity scores were assessed by a blinded dermatologist based on Physician global assessment (PGA) score. G) Representative HE images of ears. Scale bar = 500 μm. H) Total CD45+ cells in the ears were counted by flow cytometry. I) Eosinophil counts defined as total live single cell CD45+ CD11b+ Ly6G- Siglec F+ cells. J) Monocyte counts defined as total live single cell CD45+ CD11b+ Ly6G- Siglec F- F480- CD11c-. K) CD4 T cell frequencies in MC903 treated mouse ears. L) Treg frequencies in the ears and (M) pLN. N) Free iron was quantified by flow cytometry in live GFP+ Tregs from the pLN. O) Frataxin expression of Foxp3+ Tregs was determined by flow cytometry in the pLN. A, C, E, G, H, M= Welch’s t test. B, H, I, J, L, M N, O= 2 way ANOVA.

Article Snippet: WT and KO Tregs were isolated from CD4-cre + and – spleens according to the (Stem Cell Technologies) and mixed in a 1 to 3 ratio with naïve CD4 T cells isolated from WT CD45.1 mice according to the Naïve CD4 T Cell Isolation Kit (Miltenyi Biotec).

Techniques: Expressing, Flow Cytometry, Single Cell